Reference genome Mycobacterium tuberculosis. NC_00962.3 , GCF_000195955.2 , GI:448814763.
I had tried to add the above genome in database of snpEff, using the command as mentioned by the programme. It is still showing error. Any suggestions please.
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
I have 3 different specimens. Each specimen has a main peak of the targeted phase, however, all of them also have a secondary peak of associated with a secondary phase. Now just based on the...
26 June 2024 5,049 5 View
Hello! I am pursuing Ph.D. in Environmental Sciences from Sambalpur University, India. My thesis topic broadly focuses on physico-chemical analysis of water and soil samples. I eagerly want to...
18 June 2024 1,897 4 View
I am determining the alpha amylase inhibition activity of my methanolic extracts by DNSA method. The problem is, my sample absorbance is higher than the negative control which made the final...
27 May 2024 9,348 0 View
I need to use and adapt the parenting style questionnaire in my ongoing research project, can somebody please help me regarding gaining permission and access to them as I am unable to find the...
25 May 2024 6,304 1 View
I'm doing OSCC cell culture and routine media (supplemented MEM) changes every 72 hours. The media looks healthy in terms of color (reddish-orange), and there was no microbial contamination...
12 May 2024 9,279 2 View
I am using Purelink RNA mini kit (Fischer Scientific) to extract total RNA from bacteria. i need to get better RNA integrity number like more than 7, but for only one sample I got 7.4, for other...
01 May 2024 4,355 3 View
Water flows in a 2D pipe, I am able to prepare a simple simulation resulting in the velocity and pressure across the pipe. But I am interested to simulate the Wall shear stress generated by the...
22 April 2024 8,537 5 View
Achieving stable quantum computations is a challenging task due to the fragile nature of quantum systems. Welcome your valuable answers please. I am also proposing an idea to achieve this as...
21 April 2024 10,032 1 View
I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...
28 March 2024 7,798 0 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
Hello researchers, I have sequenced the A549 cell line using RNA sequencing approach and then I have it against the human genome reference sequence. Would I expect a 100% mapping percent? I got...
20 July 2024 9,705 0 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,579 2 View
We are in need of primers for a PCR protocol for chicken genome. We are looking at the COI barcode, 648-bp fragment of the 5' end of COI. Specifically Primers FalcFA, BirdR1, CO1_ExtF,...
16 July 2024 118 2 View