I don't mean to extract bone marrow cells, but the femur is supposed to keep viable for tissue culture.
Thanks, Karima!
Infrared spectroscopy measures the infrared (IR) radiation after interacting with a sample, which is directed to the spectrometer’s detector. Thus, the sample absorbs part of the IR radiation, and...
28 February 2023 6,529 3 View
It is known that cadherin proteins can show impaired function when there is no calcium in the human body due to some disease. But is it possible to have symptoms of deficiency in cadherin proteins...
19 December 2022 2,005 0 View
Dear researchers, I'm planning on quantifying ROS production using DHE superoxide indicator in human macrophages challenged with Mycobacterium tuberculosis. Due to bio-safety issues, samples need...
21 April 2017 2,662 3 View
The strain BL21(DE3) of Eschericha coli have genes that control promoters for T7 RNA polymerase, it´s possible to use vectors with T5 promoter region. This strain have genes that control a T5...
18 September 2016 9,410 4 View
I am cooling down a glass plate that is at 400C and putting it on two stainless steel supports that are initially at 25C. How to define the contact temperature of these two different bodies on...
14 April 2016 8,708 2 View
I've done some research on the topic and, so far, it was possible to identify that the following patterns have been thoroughly explored: 1) Sequence Mining. Example: "A-B-C-D happened in 10% of...
02 January 2014 5,250 11 View
During routine microscopic observation of Cancer Associated Fibroblasts (CAFs), my colleague Pedro Barcellos and I came across these odd geometric "dark" structures inside the cells. We haven't...
01 January 1970 1,696 3 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View