What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the best way to isolate proteins which are located in the endosomes and the ER membrane?

For one of my projects, I need to perform WBs on cell lysates. The protein of interest (LRAT) is located in the endosomes and the membrane of the ER. What is the best protocol to isolate proteins...

25 November 2020 969 3 View

What is your sentiment concern the impact of Covid19 on the main industries in Eu and USA ?

Covid 19 seem to be an huge crisis factor for the economy of several country . Im looking data for a first evaluation on EU and USA economy for to understand the best action for fight this impact .

07 April 2020 5,254 10 View

Why differs CT values of one sample between its triplos (Taqman Assays and qPCR)?

At first, we performed transfections. To analyze that transfection, we performed RNA isolation, cDNA synthesis and qPCR. Using the qPCR, we analyze multiple samples, whereof each sample we...

19 March 2020 2,301 4 View

What is the optimal method to the cryopreservation of SH-SY5Y cells?

I have tried multiple times to cryopreservation of SH-SY5Y cells, but afther thawing, no cells had survived. I bring the cells to cold (4 degrees Celsius) DMEM/F-12 medium with 5% DMSO. After...

14 November 2019 3,474 3 View

Why a gel-liked layer was found on the filter membrane during the ultra-filtration process for polymer nanoparticle?

Recently, I purified a polymer nanoparticle in aqueous phase using ultra-filtration (60 psi). After the filtration process, a gel-liked layer was found on the filter membrane. Dose any one know...

26 June 2018 5,803 3 View

How to choose a suitable dialysis tubing or filter membrane?

I need to separate polymer nanoparticle (hydrodynamic radius ~ 110 nm) from poly(ethyleneimine) (MW ~ 750kDa). What MWCO of the dialysis tubing or filter membrane should I choose? How can I make...

22 April 2018 3,966 3 View

What data analysis tools can be used in order to validate data quality?

I am looking for data analysis tools that can be added into a new database (legacy system migration, without database from legacy system) which takes structured data (pre-determined format, seen...

25 March 2018 269 3 View

What should I do when comparing response frequencies in multiple response items?

I have a multiple response item consisting of 10 response options. Participants were instructed to choose 3 of them. I want to know which one of those response options was picked the most. Of...

06 December 2017 6,047 6 View

How to reduce off-target effects siRNA?

28 November 2016 2,726 8 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Person-Codes for questionnaires in longitudinal studies: Is there an easy solution for better matching-rates?

Hi, I have a problem regarding matching of longitudinal data in my field of research (university students). We have a Person-Code for matching data measured in different time points, but 30%+ of...

17 February 2021 3,075 4 View

Can anyone provide an advice on running a validation study of a commercially available product in collaboration with the manufacturer?

We are planning to run a study validating a commercially available product (i.e. software) and while we initially planned to do it independently of the manufacturer, they heard about our intention...

17 February 2021 2,295 3 View

What are the latest topics in strategic management area, esp those related to Quantum managment?

I am trying to involve science , in particular Physics, into Strategic Management studies for my master thesis . There are a number of papers discussing this topic but in such a fast changing...

13 February 2021 891 5 View

What non-parametric alternative can I use for a repeated-measures ANOVA for 2 groups and 2 timepoints?

Hello, I have a question regarding non-parametric alternatives for a RM-ANOVA. In my study I had two groups that were measured on two timepoints (pre-post measurements only). I was planning to...

11 February 2021 8,599 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View