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I was trying to Immunoprecipitation Drosha protein from mice C57 BJ/6 liver. I lysis 80mg in 1.2ml lysis buffer(from thermo +proteinase inhibitor cocktail). However, after Immunoprecipitation by...

05 June 2018 7,613 3 View

Why my AAV2/8-mFoxo1 virus does not work in mouse liver?

I have a question about the AAV-Foxo1 virus. I want to know is there anybody publish a paper with AAV virus for Foxo1 overexpression? I have pack an AAV 2/8-mFoxo1 virus, which is a fusion...

10 November 2017 1,460 0 View

What's the possible explanation for inconsistance between luciferase activity and in vivo overexpression?

09 October 2017 3,883 3 View

Has anybody else encountered problems with BAC extraction during 3C protocol?

Hey guys, I was doing BAC Clone extraction for Chromosome Conformation Capture(3C). We used alkaline lysis method to lysis the bacterium. Then centrifuged and applied supernatant to DNA extraction...

11 December 2014 2,294 6 View

How can I improve the yield of BAC?

Dear friends, I have a puzzle during BAC extraction. The sample we extracted seems to be contaminated by RNA, although we digested it with RNase A and we cannot see the band for BAC in...

11 December 2014 5,574 2 View

What is the possible explanation for the different outcome between Electrophoresis and Nanodrop 2000?

09 October 2014 9,637 12 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View