Guang-Yuan Xiao

7 Questions 10 Answers 0 Followers

Questions related from Guang-Yuan Xiao

During immunoprecipitation， I can see my target proteins in inputs, but not in immunoprecipitation, why?

I was trying to Immunoprecipitation Drosha protein from mice C57 BJ/6 liver. I lysis 80mg in 1.2ml lysis buffer(from thermo +proteinase inhibitor cocktail). However, after Immunoprecipitation by...

06 June 2018 7,664 3 View

Why my AAV2/8-mFoxo1 virus does not work in mouse liver?

I have a question about the AAV-Foxo1 virus. I want to know is there anybody publish a paper with AAV virus for Foxo1 overexpression? I have pack an AAV 2/8-mFoxo1 virus, which is a fusion...

11 November 2017 1,537 0 View

What's the possible explanation for inconsistance between luciferase activity and in vivo overexpression?

Hey， Guys, I'm trying to identify a target gene for Foxo1 in Huh7 cell line. It's very strange that we have EMSA , luciferase promoter increase. However, when we over express Foxo1 in Huh7 cell...

10 October 2017 3,931 3 View

Does anyone know any database we could use to search profile of protein expression level of the C2C12 cell line?

I want to know the protein expression profile of C2C12 cellline, which may help me to analyze some problem occured during experiment.

12 December 2014 4,979 3 View

How can I improve the yield of BAC?

Dear friends, I have a puzzle during BAC extraction. The sample we extracted seems to be contaminated by RNA, although we digested it with RNase A and we cannot see the band for BAC in...

12 December 2014 5,642 2 View

Has anybody else encountered problems with BAC extraction during 3C protocol?

Hey guys, I was doing BAC Clone extraction for Chromosome Conformation Capture(3C). We used alkaline lysis method to lysis the bacterium. Then centrifuged and applied supernatant to DNA extraction...

12 December 2014 2,348 6 View

What is the possible explanation for the different outcome between Electrophoresis and Nanodrop 2000?

I performed a plasmid mini extraction for pEGFP.N2 last time. And I found that in electrophoresis in 1% agar gel, concentration of sample 1 and sample 2 were equal (their brightness is the...

10 October 2014 9,693 12 View