Please provide references to papers in which alpha-synuclein concentration is estimated or to papers in which a suitable method to quantify protein concentration in neurons is applied.
http://www.sciencemag.org/content/342/6161/983.long#F2
Published Online October 24 2013
Science 22 November 2013:
Vol. 342 no. 6161 pp. 983-987
DOI: 10.1126/science.1245296
REPORT
Identification and Rescue of α-Synuclein Toxicity in Parkinson Patient–Derived Neurons
Thanks for the answer. I had a quick look to the paper, but I could not find a precise estimate of alpha-synuclein concentration in neurons. Could you provide this number?
Anyway, I will read it more carefully during the week end and I will look also at the Supplementary materials. Thanks again.
another one, some numbers from ELISA and histopathology data http://www.sciencedirect.com/science/article/pii/S0014488608002562
or you can contact directly
Prof. Tiago Fleming Outeiro
Phone: ++49-551-3913544
Fax: ++49-551-3922693
e-mail: [email protected]
http://www.neurodegeneration.uni-goettingen.de/index.html?en/p211.html
According to this paper, it's 6uM: http://www.sciencedirect.com/science/article/pii/S0092867412004710
Thank you very much for the reference. I had a look at the reference provided in the paper you cited and it seems that this estimate comes from western blot analysis or something like that. I think that this is not the best method to evaluate alpha-synuclein concentration in neurons. But this result is what can be found in the literature. Therefore, thank you very much for your reply.
Should you send me the paper please i dont have account and i need to read it as i am running a model of PD with rotenon now. and have a problem as i have a high mortality rate with 1.5mg/kg rats PEG:DMSO 1:1
- Badges
- Science topic
- Similar topics
- Biological Science
- Neuroscience