Hani Hafez

10 Questions 16 Answers 0 Followers

Questions related from Hani Hafez

Greeting, The bufo phylogenetic tree

Dear All, The construction of phylogenetic trees using NJ or ML can be constructed without Outgroup. the used genes are very difficult to find out outgroup. The trees are for D loop and 12s Genes...

10 August 2017 8,238 1 View

How can I construct one phylogenetic tree using two genes together?

1- I have sequences for different species of Bufo, these sequences are for two genes control region and 12s. I am asking if i can construct one phylogenetic tree using both gene or it is better to...

02 December 2015 286 6 View

What is the best antibody to detect the aggregates of alpha synuclein in rats injected with rotenone, primary and secondary Ab for IF, please?

I want to know, what is the best antibody to detect the oligomerization of phosphorylated alpha synuclein aggregates in rats injected with rotenone. if it is possible the company name and the...

10 March 2015 7,131 3 View

How can I avoid high mortality of rotenone-induced PD?

I used 1.5mg/kg dissolved in DMSO/PEG 1:1 and I have high mortality intraperitoneal injection.

31 October 2014 4,292 1 View

What are the principal instruments for construction of a neurodegeneration study lab?

I want to construct new lab in neurodegeneration using transgenic animals and cell lines and I need to know the most important for the studies such as fluorescence and other main used devices.

15 May 2014 7,521 3 View

What is the best dose of rotenone to induce PD in rats in the case of intravenous injection?

Is it better to dissolve rotenone in DMSO:PEG 1:1 or one of them only and what is the best concentration of them to be used if I will use them separately?

10 May 2014 3,502 0 View

Any Indian professor working in the field of neurogenesis and stem cells who also has developmental biology interests?

I'm looking to collaborate.

07 April 2014 9,575 1 View

Is there any other factor for the induction of Parkinsonism with rotenone? I inject it 1.5 1:1 PEG and DMSO, but result in a high mortality rate.

problem in the injection of the rotenone to induce PD. I inject it 1.5mg/kg 1:1 PEG and DMSO, which results in a high mortality rate.

19 March 2014 1,998 3 View

How can I estimate the sequence of the SiRNA that will be used?

I want to prevent the expression of 11 amino acid in the peptide chain using siRNA,

17 February 2014 5,628 2 View

If I want to calculate the MRCA for Bufo arabicus using RelTime, what calibrated taxa do I have to select?

And what is the divergence time I have to use? Thanks in advance.

04 February 2014 434 1 View