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Questions related from Rita Assenza
Hi, I am looking for an assay (simple and fast) to quantify a 12AA peptide quantification inside PLGA nanoparticles. I did a PLGA/peptide solution in DMSO and after that I used F127 as surfactant....
21 September 2021 3,505 1 View
My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...
04 March 2020 8,169 4 View
Hello. I treated the 3D spheroids with a molecule FITC-conjugate. After 5 days of colture, I would like to fix the spheroids to see the fluorescence at confocal microscopy. Do you a method to...
11 February 2020 9,106 0 View
Hello! I am trying to measure calcium concentration into Sarcoplasmic reticulum from adult mouse heart. SR vesicles were obtained from male mice (8-12 weeks old), by several centrifugations in...
15 April 2018 5,961 1 View
Hi, is it possible to quantify the intracellular calcium concentration (in adult mouse cardiomyocytes) using the Ca2+ indicator Fluo-4? Thank you very much.
12 February 2018 6,323 3 View
Hi, I was wondering if anyone would know of a simple method to measure the calcium free in Sarcoplasmic Reticulum and/or the calcium bind to Calsequestrin in mouse adult cardiomyocytes. Thank you...
11 February 2018 7,444 0 View
Hello, how can I measure Sarcoplasmic Reticulum Calcium concentration in adult cardiomyocytes?
12 November 2017 5,158 3 View
Hi. One year ago I stained paraffin-embedded tissue sections (embryo mouse heart) with antibody anti-ki67 (SantaCruz) for immunofluorescence. I tried to strip and reprobe these sections with...
09 December 2015 2,484 4 View