Rita Assenza

3 Questions 3 Answers 0 Followers

Questions related from Rita Assenza

Does anybody have an idea of how to solubilize hydrophobic peptides?

My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...

04 March 2020 8,143 4 View

Is it possible to measure SR Ca2+ concentration by colorimetric methods?

Hello! I am trying to measure calcium concentration into Sarcoplasmic reticulum from adult mouse heart. SR vesicles were obtained from male mice (8-12 weeks old), by several centrifugations in...

15 April 2018 5,940 1 View

Is it possible to strip and re-probe paraffin-embedded tissue sections for immunofluorescence?

Hi. One year ago I stained paraffin-embedded tissue sections (embryo mouse heart) with antibody anti-ki67 (SantaCruz) for immunofluorescence. I tried to strip and reprobe these sections with...

09 December 2015 2,473 4 View