Rita Assenza

8 Questions 4 Answers 0 Followers

Questions related from Rita Assenza

Does anyone know an assay for peptide quantification into PLGA nanoparticles?

Hi, I am looking for an assay (simple and fast) to quantify a 12AA peptide quantification inside PLGA nanoparticles. I did a PLGA/peptide solution in DMSO and after that I used F127 as surfactant....

21 September 2021 3,504 1 View

Does anybody have an idea of how to solubilize hydrophobic peptides?

My hydrophobic peptide (12 AA) is soluble in 100% DMSO. Given that I should encapsulate it in hyaluronic acid nanoparticles, I need that peptide is soluble or partly soluble in water. Does someone...

04 March 2020 8,167 4 View

How to fix spheroids to avoid loss of FITC signal ?

Hello. I treated the 3D spheroids with a molecule FITC-conjugate. After 5 days of colture, I would like to fix the spheroids to see the fluorescence at confocal microscopy. Do you a method to...

11 February 2020 9,104 0 View

Is it possible to measure SR Ca2+ concentration by colorimetric methods?

Hello! I am trying to measure calcium concentration into Sarcoplasmic reticulum from adult mouse heart. SR vesicles were obtained from male mice (8-12 weeks old), by several centrifugations in...

15 April 2018 5,959 1 View

Is it possible to quantify the calcium through Fluo-4?

Hi, is it possible to quantify the intracellular calcium concentration (in adult mouse cardiomyocytes) using the Ca2+ indicator Fluo-4? Thank you very much.

12 February 2018 6,322 3 View

How to test for Ca(2+) free versus Calcium bind to Calsequstrin?

Hi, I was wondering if anyone would know of a simple method to measure the calcium free in Sarcoplasmic Reticulum and/or the calcium bind to Calsequestrin in mouse adult cardiomyocytes. Thank you...

11 February 2018 7,443 0 View

How to measure SR Ca2+ concentration in cardiomyocytes?

Hello, how can I measure Sarcoplasmic Reticulum Calcium concentration in adult cardiomyocytes?

12 November 2017 5,157 3 View

Is it possible to strip and re-probe paraffin-embedded tissue sections for immunofluorescence?

Hi. One year ago I stained paraffin-embedded tissue sections (embryo mouse heart) with antibody anti-ki67 (SantaCruz) for immunofluorescence. I tried to strip and reprobe these sections with...

09 December 2015 2,482 4 View