Dear Stephen Holt

I tried this dose but more than 50% of animals died after 24 hours. I wnat to know this rate of death after administration of glycerol is usual result? or there are some problems in my experiments maybe in administration of glycerol or something elss.

Thank you in advance.

Stephen Holt's answer is correct. To add to that, you may need to dehydrate the animals prior to injection (we generally used Sprague-Dawley rats and restricted access to water for 12 hrs prior to injection). We also had zero mortality in rats but some mortality in mice with this model. You could try a lower dose (i.e. 8 ml/kg) if the injury is too severe. See Richard Zager's work, he has used this model extensively, and I think he has published work on the low and high doses. Karl Nath is also a good reference for this model.

I used Sprague-Dawley rats and ketamine/Xylazine procedure to anesthetize animals. As Funk said I think I should try the lower dose of glycerol.

According to my experiments, 24h after glycerol injection, the serum bun and creatinine levels increase to 413mg/dl and 4.3 mg/dl respectively. Are these amounts close to your results?

Thank you for the help

Before glycerol injection animals were dehydrated for 18 hours, maybe it is long time and I should shorten it. IP injection of saline, could affect serum bun and Cr levels?

Dear Stephen Holt

Thank you , I try again with consideration of your advices.

Fatemeh, I use 6 mL/kg of 50% v/v solution of glycerol in male Wistar rats. Other thing that can help: test the model without the dehydrate first. I made groups of 120 hours. Injection of saline it's not a good control or sham group, because the i.m. injection of pure saline (i.e. 6 mL/Kg) can induce skeletal muscle injury and kidney injury also.

Hi.. Can you guys give me the relevant references for following this model