Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,277 6 View

Tool to calculate lag between BOLD time series?

Dear community I would like to ask if you are aware of any tool (Matlab/toolbox, etc.) that calculates the "lag between BOLD time series", as described in the following publications:...

01 November 2018 2,428 2 View

Is it necessary to make students prepare class reports as well as insect collections?

Dear All, I need the experience, data and opinion of zoologists/entomologist teaching zoology/entomology for BSc or MSc courses. It is necessary to make students prepare reports on the...

18 May 2017 7,405 15 View

T47D and A2780 growth problem.Why?

We work for many years with different gynecological cancer cell lines with the usual conditions. Two of these are T47D (breast) and A2780 (ovarium). For a month, this two cell lines are growing...

26 March 2017 5,358 7 View

Is there any side effects of eating rice or grains with read meat?

Red meat is harder to digest than for example poultry. If you eat a high carb meal with red meat the higher ptyalin production can increase the pH in the stomach resulting in bad digestion. Is...

03 March 2017 2,602 10 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,734 2 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

No title

01 March 2016 5,806 6 View

Permanova versus adonis, difference between estimates of components of variation and explained variance?

I try to compare the output from permanova (run in PrimerE) and adonis (in R) on the same dataset. SS, MS, and F are identical. However, I am getting more and more confused about the terms...

09 December 2015 7,825 4 View

In MTT assay a cancer cell line growth activity (untreated control cells) is decreased in repeated measurements. Why?

We usually use MTT assay with more cervix carcinoma cell lines to detect antiproliferative effect of drugs. At the end of these measurements we get absorbance values according to produced formazan...

13 October 2015 9,429 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View