Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,277 6 View

Tool to calculate lag between BOLD time series?

Dear community I would like to ask if you are aware of any tool (Matlab/toolbox, etc.) that calculates the "lag between BOLD time series", as described in the following publications:...

01 November 2018 2,428 2 View

Is it necessary to make students prepare class reports as well as insect collections?

Dear All, I need the experience, data and opinion of zoologists/entomologist teaching zoology/entomology for BSc or MSc courses. It is necessary to make students prepare reports on the...

18 May 2017 7,405 15 View

T47D and A2780 growth problem.Why?

We work for many years with different gynecological cancer cell lines with the usual conditions. Two of these are T47D (breast) and A2780 (ovarium). For a month, this two cell lines are growing...

26 March 2017 5,358 7 View

Is there any side effects of eating rice or grains with read meat?

Red meat is harder to digest than for example poultry. If you eat a high carb meal with red meat the higher ptyalin production can increase the pH in the stomach resulting in bad digestion. Is...

03 March 2017 2,602 10 View

In what deeper sense can you speak about the quasiclassicality of the DNA macromolecule?

You can see under an ordinary light microscope that a DNA polymer is nearly a macroscopic object. But in a strict quantal sense can we expect quasiclassical behaviour, i.e., describability by a...

03 February 2017 7,734 2 View

Does anyone know about a Gene Expression Library (RNA-Seq), containing gene expression values across different cell-types and cell lines?

The ones I could find so far are highly biased towards cancer, and I needed a representative library. Does anyone know about such resource? Thanks

09 January 2017 122 3 View

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm. Can anyone help?

I would like to ask for 2D potentiometric SECM images to use them as examples for my deconvolution algorithm in my doctoral thesis. I would acknowledge and reference any provided SECM data. The...

30 May 2016 1,666 2 View

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01 March 2016 5,806 6 View

In MTT assay a cancer cell line growth activity (untreated control cells) is decreased in repeated measurements. Why?

We usually use MTT assay with more cervix carcinoma cell lines to detect antiproliferative effect of drugs. At the end of these measurements we get absorbance values according to produced formazan...

13 October 2015 9,429 6 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

I am thinking of electrifying the street food carts while harvesting energy using solar panels on top.

22 February 2021 4,323 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What time and speed should the centrifuge be set at in order for cell debris and clumped proteins to form a pellet?

- The Eppendorf tube was then placed in a microcentrifuge. Notice how it is balanced with another tube placed opposite it in the centrifuge drum to ensure smooth rotation. Other information...

24 January 2021 2,547 3 View

Is Handheld XRF analyzer useable? and accurate in geochemical test?

What is the different between Lab XRF and Hanheld XRF, is it accurate and useable to analyze sedimentary rocks?

23 January 2021 9,941 6 View