I am looking for a toolbox (open source would be perfect) for 3D segmentation of vein from MRA images. Additionally, which technique is better for such segmentation PC-MRA, or TOF-MRA? Should I use any special acquisition parameters?
It all depends on your actual application, but I would be cautious about TOF based techniques due to potential areas of signal fall out due to flow direction changes, positional effects, etc. Contrast based techniques are likely to be much more robust, but you may need to work harder to remove the arterial component.
Aleksandra, check out the ITK toolkit (www.itk.org). This requires programming to use so I'm not sure if it would meet your needs. ITK is also the basis for 3D Slicer. There may be a Slicer module for vessel tracking, there are definitely plenty of segmentation tools in general that would do the job for you.