Is there any chemical or surgical method to induce hypoxia in mouse model?

Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...

15 December 2020 866 6 View

Acetic acid in liquid waste ?

Hy, may you suggest a method to quantify acetic acid in wastes or leaching test? Thanks in advance Stefano

10 December 2020 4,023 2 View

Which points I have to take in mind to mutate a plasmid that grow in Neb stable strain?

Several kits that allow the introduction of point mutations in plasmids say to transform mutated plasmids in DH5alpha bacteria. I have a plasmid with many repetitions that need stable strains to...

08 November 2020 4,250 2 View

Did you have any problem for staining nuclear phosphorylated proteins in hippocampal neurones?

I was wondering if there may be a problem of permeabilisation of cells. I am working on mice hippocampus (pups) and I need to make a staining for a phoshoprilated nuclear protein. Since the...

17 September 2020 2,000 3 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How many researchers are interested in attending web communications in the field of Sustainable Planning?

17 June 2020 4,753 74 View

What happens to non-"preclincial essential research" (non Covid19) in Covid times?

Are you askiing what researchers are doing in Covid time especially those that work with animal? I started a survey to collect information (anonymous) to see whether there are alternatives to...

02 April 2020 9,596 3 View

Generic DC/HYBRID grid-connected microgrid available in simulink?

I'm looking for a microgrid simulation model written in simulink in order to implement my simulation data and test my Energy Management System algorithm. The microgrid I am looking forward is a...

24 October 2019 3,541 4 View

Is there an investigation about the real world implementation of decision making algorithms in smart home/microgrids EMS microcontrollers?

Implementation of stochastic and probabilistic based algorithm is a very tricky task especially in energy management systems microcontrollers. I'm looking for a research paper which talks about...

30 July 2019 5,947 1 View

What is the best choice for a ribosomal protein used as a loading control in WB?

Hi everyone. I have to assess protein expression in my adult satellite muscle cells by WB. I'd prefer not to use the conventional beta-actin or alpha-tubulin, nor GAPDH. I saw that 18S mRNA was...

24 June 2019 3,133 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View