Dear Harpreet Kaur Ranjit Singh

please see the link below from Miltenyi Biotec, which nicely lays out defining markers for eosinophils and basophils, isolation protocols from PBMC and flow cytometry plots, which should help your gating strategy.

https://www.miltenyibiotec.com/CA-en/resources/macs-handbook/human-cells-and-organs/human-cell-types/granulocytes-human.html

All the best & kind regards,

Michael

Michael Förster this is really helpful. Thank you very much!

Hi

This ismay be a useful link: https://www.biolegend.com/en-us/essential-markers

BW

Stephen