Harpreet Kaur Ranjit Singh

4 Questions 2 Answers 0 Followers

Questions related from Harpreet Kaur Ranjit Singh

Probe based primers for conventional PCR?

I’ll be doing qPCR using probe based primers. The primers and probes are premixed. I’ve heard that before optimising qPCR, a thermal gradient PCR has to be done. Could I do a thermal gradient PCR...

28 August 2020 2,094 2 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

09 March 2020 9,731 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

07 January 2020 3,861 3 View

How much blood would I need to culture T cells in a T-75 flask?

I will be isolating T cells from blood samples and culturing them in a T-75 flask. What would be the required blood volume needed? Or rather, how much blood would I need to have enough of cells to...

22 July 2019 5,632 1 View