Harpreet Kaur Ranjit Singh

6 Questions 3 Answers 0 Followers

Questions related from Harpreet Kaur Ranjit Singh

Probe based primers for conventional PCR?

I’ll be doing qPCR using probe based primers. The primers and probes are premixed. I’ve heard that before optimising qPCR, a thermal gradient PCR has to be done. Could I do a thermal gradient PCR...

28 August 2020 2,217 2 View

Hi can someone share a protocol for manual DNA extraction from frozen cell pellet?

Planning to extract DNA from frozen cell pellets but I do not have a kit. Anyone has a protocol for manual extraction? Thank you!

27 July 2020 1,807 0 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

09 March 2020 9,840 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

07 January 2020 3,969 3 View

How much blood would I need to culture T cells in a T-75 flask?

I will be isolating T cells from blood samples and culturing them in a T-75 flask. What would be the required blood volume needed? Or rather, how much blood would I need to have enough of cells to...

22 July 2019 5,680 1 View

Hello, I was just wondering why HEK-293 cell line is used widely in Influenza virus studies instead of healthy lung cells. Any thoughts?

I know many Influenza studies use HEK-293 and A549 cell lines instead of healthy lung cells since influenza infects the respiratory tract. Was just wondering the reason to this.

05 September 2017 1,473 2 View