Does anyone know a specific inhibitor of Exportin-5? miRNA transport
Dear Vincenzo,
Please see the following:
Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels
Yamina Bennasser, Christine Chable-Bessia, Robinson Triboulet, Derrick Gibbings, Carole Gwizdek, Catherine Dargemont, Eric J Kremer, Olivier Voinnet & Monsef Benkirane
Nature Structural & Molecular Biology 18, 323–327 (2011) doi:10.1038/nsmb.1987
Received 11 February 2010 Accepted 23 November 2010 Published online 06 February 2011
Abstract
Abstract•
References•
Author information•
Supplementary information
MicroRNAs (miRNAs) are a class of small, noncoding RNAs that function by regulating gene expression post-transcriptionally. Alterations in miRNA expression can strongly influence cellular physiology. Here we demonstrated cross-regulation between two components of the RNA interference (RNAi) machinery in human cells. Inhibition of exportin-5, the karyopherin responsible for pre-miRNA export, downregulated expression of Dicer, the RNase III required for pre-miRNA maturation. This effect was post-transcriptional and resulted from an increased nuclear localization of Dicer mRNA. In vitro assays and cellular RNA immunoprecipitation experiments showed that exportin-5 interacted directly with Dicer mRNA. Titration of exportin-5 by overexpression of either pre-miRNA or the adenoviral VA1 RNA resulted in loss of Dicer mRNA–exportin-5 interaction and reduction of Dicer level. This saturation also occurred during adenoviral infection and enhanced viral replication. Our study reveals an important cross-regulatory mechanism between pre-miRNA or viral small RNAs and Dicer through exportin-5.
For full paper, see attached file.
hoping this will be helpful,
Rafik
Thanks Rafik. I had already seen the pope that you have reported.However I was looking for a type of chemical inhibition and not through siRNA . Thanks anyway.
Vincenzo
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