We used Sperm Freezing medium for T. brucei with great success!

You could try it: for cruzi: "A HEPES-buffered salt solution containing 10 mg/mL human serum albumin, gylcerol and sucrose as the cryoprotective medium, and gentamicin as an antibiotic".

Good luck!

We simply use LIT with 5% DMSO, put them in cryotube for one night at -80 and then put in liquid nitrogen.

We use 50% BFS, 40% T.cruzi in LIT medium and 10% glycerol. In a cryotube overnight at -80 and than in nitrogen.

you can try freezing with 50% glycerol. The total volume should be around 1ml. The initial cell population before freezing for T.brucei is maintained between 8e6 to 1e7 cells/ml. You may try without centrifuging the cell suspension.

In a cryovial add 800ul of cell suspension and then add 200ul of 50% glycerol.. Mix it properly and immediately put in ice. Transfer it to -70C.

On the next day or two, transfer the vial to liquid Nitrogen.

You can try this protocol for one set of your cells to see if it works in your cell line.

Thank you all

Whatever you choose, if you opt for a protocol containing DMSO, keep in mind it's a killer; you've got to remove it/ dilute it strongly as fast as possible.

By saving one of these parasites is the freezing of the parasite and the provision of food items would be put and the amount of rabbit serum with Ksrol faithful and the development of the parasite

By freeze-fracture technique?

RPMI media + 10 % FBS + 5% DMSO should work well for cryopreservation. But after adding all, place it at -80 degree C for overnight and then shift to -196 degree celcius. Best wishes.!

Provide a suitable environment for the parasite to survive and by freezing