20 April 2015 5 7K Report

I can't purchase the one from abcam in Australia.

More Jeremy FP Ullmann's questions See All
What plasmid type that you get from isolation of a culture?

Greetings for all of scientist using this platform. I have a little problem. Recently I had done reconstruction of my plasmid (Named PDR111, length = 11,8 kb). Transformed culture i named it W1...

21 April 2024 7,921 4 View

Related how to Anneling in Silvaco TCAD ?

When I simulate the NMOS model in ATHENA, when I want to anneal in nitrogen atmosphere after n+ ion implantation, the annealing operation is not possible when the annealing temperature is lower...

08 April 2024 2,798 0 View

How to confirm your ligation before its transformation in E.coli?

So let say I have circular plasmid named "PDR111". I did PCR with KOD ONE MIX KIT and the product is linear DNA. Then, I did self circularization of linear DNA with T4 DNA Ligase protocol (Thermo...

11 March 2024 1,143 7 View

Are there any established methods to map and compare health and social care services across two or more different areas?

I am involved in a planning a research project. We would like to map how health, social care and voluntary services are provided for a particular practice specialism. Do people know of any...

05 April 2023 1,332 1 View

Flight Planning Apps for Parrot ANAFI Ai Drones?

I am curious what flight planning apps researchers are using to preplan and fly flight missions for Parrot ANAFI Ai drones. Are there any good options besides the FreeFlight 7 app?

13 March 2023 491 4 View

How to plot SHmax directions (borehole breakout) on ArcGIS?

Does anyone know how to plot SHmax directions (borehole breakout) on ArcGIS?

01 March 2023 4,463 2 View

What is the best technique/mixing method for drug combos?

Hi All, I have previous experience with devising HTDS and drug combinations, however, that was carried out using very specialized HTS machines using vibrations etc. Now, I am screening fewer...

14 February 2023 4,558 0 View

What statistical method can be used to correlate Leptospirosis morbidity rates to Precipitation, Land Surface Temperature, NDVI?

We are currently trying to find out which climate and/or urbanization factors correlate with Leptospirosis in the Philippines. For this, we have gathered precipitation, land surface temperature,...

23 January 2023 8,966 2 View

Is Land Surface Temperature always Correlated with Precipitation?

We are currently doing correlation with Land Surface Temperature (LST) and Precipitation data from Landsat (Top of Atmosphere) and CMORPH respectively. However, it seems that for the four cities...

23 January 2023 7,155 3 View

Ma-mel-66a cell line culturing methods?

Hello, I have been trying to passage ma-mel-66a cell line, however, I am having zero luck. I have used RPMI + 10%FBS and RPMI + 5%FBS, I even plated them (straight from LN2) in a small p10 to...

10 January 2023 9,305 5 View

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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Can I use a HisTRAP column for affinity chromatography?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

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How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

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How to isolate lymphocytes from mouse spleen?

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