Hello Yogesh,

Membrane blabbing is one of the morphological features characteristic of apoptosis commonly observed under in vitro culture conditions in various somatic cell types as well as in germ cells like oocytes under stressed or drug-induced conditions.

Hypertonic treatment to the Xenopus oocytes could induce a osmotic stress as the osmolarity of Xenopus oocytes is normally 240mOsmol. The hypertonic conditions will generate the hypertonic shock that may induce in the reduction of size due to oocyte shrinkage involving exocytosis and the other related to an ionic current. For detail see the link given below:

Release of ATP induced by hypertonic solutions in Xenopus oocytes. J Physiol. 2003 Feb 15; 547.

Article Release of ATP induced by hypertonic solutions in Xenopus oocytes

Membrane blabbing are seen in oocyte undergoing shrinkage during apoptosis in experimentally induced conditions and apoptosis under in vitro culture conditions.

Although we have not generated membrane blabbing in xenopus oocytes but we have observed membrane blabbing in rat oocytes undergoing apoptosis under in vitro cultured conditions.

The sequential development of membrane blabbing in rat oocytes cultured in vitro conditions can bee seen along with the detail protocol in our published paper link given below:

Hydrogen peroxide modulates meiotic cell cycle and induces morphological features characteristic of apoptosis in rat oocytes cultured in vitro. Apoptosis 2005; Aug;10(4):863-74.doi: 10.1007/s10495-005-0367-8.

Article Hydrogen Peroxide modulates meiotic cell cycle and induces m...

Hope this helps

Best