Yogesh Pandey

3 Questions 2 Answers 0 Followers

Questions related from Yogesh Pandey

Does anyone here have some experience with preparation of Xenopus laevis blebs?

Dear community, I am interested in creating plasma membrane blebs from Xenopus oocyte by hypertonic treatment. Does any one have experience in doing so? The most important question would be to as...

01 April 2022 3,905 1 View

Does anyone have experience using nitrogen cavitation for disruption of Xenopus oocytes (or cells with similar size/volume)?

Dear all, I am interested in learning about the parameters required for nitrogen cavitation of Xenopus l. oocytes. The aim is to create homogenous vesicles. Any help in the matter would be...

09 March 2022 2,021 1 View

IVT for microinjections: HiScribe vs. mMessage mMachine T7 kit?

Dear community, Does one have any experience with using both the mentioned kit and have noted any significant difference in performance? The HiScibe kit from NEB is definitely the cheaper option...

06 October 2021 5,751 3 View