I have used universal primers for bacteria but it did not work well.
Hi, Leandro
I recommend that you follow the links below to have a more comprehensive idea of your question.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://www.researchgate.net/post/Is_there_universal_primer_set_for_lactic_acid_bacteria_detection
https://eurekamag.com/pdf/004/004304831.pdf
Hi, Leandro.
Can you give us some more information like reaction reagents and thermal cycling, at least? Did you have any results or none at all?
Hi, Leandro
I recommend that you follow the links below to have a more comprehensive idea of your question.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://www.researchgate.net/post/Is_there_universal_primer_set_for_lactic_acid_bacteria_detection
https://eurekamag.com/pdf/004/004304831.pdf
Hi Adriano Costa de Alcantara
We are doing the PCR and sequencing with partner laboratories, however the results are not being satisfactory since they are using universal primers for bacteria (27F/1492R and 518F/800R).
- Badges
- Science method