Does anyone have the reference of a commercial antibody againt Twist working in Xenopus embryos?

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Hanqing Xie

Labome VAD database does not indicate that any TWIST1 antibody has been used in Xenopus embryos. If you can not find any reference, you might check with Abcam about ab50887. It has been used very extensively. Based on Abcam website, it is made with a recombinant human fragment, it can be a good candidate for you if this fragment is shared between human and Xenopus.

Russell Linscott

LifeSpan Bio indicates that their rabbit anti-TWIST pAb is cross-reactive with Xenopus, based on BLAST analysis of the immunogenic peptide. Here is their datapage:

https://www.lsbio.com/antibodies/anti-twist1-antibody-twist-antibody-c-terminus-wb-western-ls-c30601/29070?trid=0

Hope this is useful.

Eric Theveneau

Thanks guys. I will check this out.