Does anyone have the mycotoxin regulatory limits for animal feed in the UK?

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Starch analysis - Ewers is 10520 method - elevated starch results?

Hi, I am getting elevated results in my starch analysis. It is about 2-3 % higher than usual. We have not changed anything. We are getting the same results over and over. I have checked the...

25 March 2024 1,395 6 View

Functional/non specific disulphide bond formation in proteins?

As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...

21 March 2024 3,803 1 View

Protein precipitates on purification??

I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...

20 March 2024 9,095 4 View

Multiple bands detected in SDS sample of monomer species (gel filtration fraction)??

I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...

19 March 2024 4,202 3 View

Colorimetric glycoalkaloids in potato?

Hi, Has anyone got a method to test glycoalkaloids in potato. I don't have an HPLC so I'm looking for a wet chemistry method. I would like to quantify the results. If possible, I want to look a...

13 November 2023 1,668 1 View

Peroxide value and free fatty acid control?

We use an I house sample as a control sample (set up by running a PT sample along side it). However, over a few months it becomes rancid and we have to start the process again. Does anyone know of...

27 July 2023 8,679 0 View

Does anyone know of any research around a recursive learning cycle that promotes contextual learning and flexible thinking in Elementary Education?

Currently exploring similarities around the Design Thinking, Inquiry Learning, and Mathematical Modelling framework.

17 May 2023 8,746 1 View

Best way to inflate the pancreas for mouse islet isolation?

as stated

18 February 2023 7,264 1 View

Binding affinity (KD) of DNA oligos for their complement?

Folks, Does anyone know of a paper showing binding affinity data of DNA oligos for their complement? Ideally it would show data from a 'non-molecular biology method' such as ITC, SPR, or MST. Even...

12 January 2023 2,965 2 View

Can I still use a chi-square/ Fisher's exact test if the following two conditions are not met?

Can you use the chi-squared test if subjects from the groups in the predictor variable can appear multiple times in the response variable categories? & Can you use chi-square if you do not...

21 July 2022 7,061 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Is it possible to estimate population using animal footprint?

I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...

18 July 2024 3,729 7 View

Phengaris rebeli on Picea abies?

Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...

15 July 2024 8,099 4 View

Any suitable Animal models to evaluate Mast cell stabilizers?

I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.

14 July 2024 779 0 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

¿Qué podemos hacer para proteger a los Cetáceos?

Hoy me dirigí a la playa San Naplo, Perú una playa muy hermosa y con una biodiversidad increíble, el clima estaba nublado y un poco húmedo, me encontré con un hallazgo increíble y a la vez muy...

22 June 2024 1,714 0 View

Sorghum bicolor accumulates heavy metals?

If Sorghum bicolor is an accumulation of heavy metals, how are they used as fodder and food for animals?

17 June 2024 8,140 1 View