Which sequence could induce higher immunogenicity, S polypeptide or only S1? and how to isolate S1 from a vector containing the whole seq. of S?

I have a clone of DNA sequence encoding the full spike protein (S1+S2)

01 January 2021 7,821 1 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is a good pretext task for self supervised lane detection in autonomous driving?

I have been looking into self-supervised methods in computer vision. For example Digging Into Self-Supervised Monocular Depth Estimation which looks at three consecutive frames of video, tasks a...

23 July 2020 8,232 1 View

What are some ways (aside from: a. mouse weight b. girth) to measure tumor growth in mice?

We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...

25 March 2020 5,991 2 View

Can anyone help me to find the psychometric characteristics for the JCQ VERSION 2 developed by Karasek??

I am using Job demands & control model in my thesis, and I want to use JCQ 2 questionnaire, but i cant find the psychometric characteristics

08 February 2020 2,240 3 View

Can we use a systemic NOAEL of a chemical product in a oral-administration effects study ?

Good evening, I have a already esablished Overall Systemic NOAEL (mg/Kg bw/day) for a pesticide wich i'll be studing the effect by oral administration on rats, can i use directly this dose in my...

08 November 2019 8,265 1 View

References about GA convergence or non-convergence?

I am looking for references to show that GA solutions do not necessarily converge to optimal solution to defend the use of an integer program, exact solution. I want to criticize heuristic and...

05 August 2019 900 5 View

In Data Envelopment Analysis (DEA), what to do if you are comparing with respect to outputs only?

Can we do that? And if yes, assuming that we have negative and positive output features, how to deal with them? By negative and positive features I do not mean value, but a feature like happy and...

22 January 2019 2,068 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View