Does anyone have suggestion for the house keeping gene to be used in qRT-PCR of bacteria, Salmonella?

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Hello every one. I need to convert the DSC results from micro volt to joule.how?

hello every one. I need to convert the DSC results from micro volt to joule.how?

04 September 2023 4,813 7 View

How to calculate the UV dosage in wastewater treatment in mJ/cm2?

I have a UV lamp (Philips 15 W, 254nm). The measured total Radiated Power was 5.3 Watts. The lamp is located vertically inside the reactor, and the wastewater is around the lamp. I want to...

28 June 2023 775 1 View

What is the range of overall heat transfer coefficient in economizers?

the hot fluid is flue gas at shell side, which is mixed, the cold fluid is water at finned tube side which is unmixed?

26 June 2023 1,850 2 View

The method fo measuring GSH levels, GST, GPx and catalase activities in plasma please ?

03 May 2023 7,043 1 View

Where can i find a dataset for emotion/arm movement recognition using an EEG Headset?

I need a dataset for emotions or arm movement recognition such as "Happy", "Sad", "Angry" etc. or "Left", "Right", etc. through the use of a Neurosky headset or as long as it contains the...

28 May 2022 7,516 1 View

I want to measure the impact of changing in laws and regulations on the market capitalization (value) of stock exchange and I want to use?

I want to measure the impact of changing in laws and regulations on the market capitalization (value) of stock exchange and want to use dummy variables, what is the best model to do that. I mean...

07 February 2022 2,092 3 View

Business Strategy and strategy plan ?

I need research papers or books related to theories of business strategies and best practice for strategy plan

04 January 2022 6,057 5 View

Best open access journal for pediatric nutrition?

What is the best open access journal for a pediatric nutrition manuscript...? Any past experience?

05 December 2021 1,550 7 View

What is the appropriate wavelength for measuring Streptomyces spp growth ?

What is the appropriate wavelength for measuring Streptomyces spp growth

02 September 2021 9,156 2 View

Convert platform IDs to gene symbol for GSE111636??

Can someone lend insight in how to convert platform IDs to gene symbol for GSE111636?? Typically, I have been able to get away with converting microarray ids from geo datasets to gene symbols...

31 March 2021 7,924 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View