Does anyone have success with extracting yeast using the Qiagen DNeasy PowerSoil Pro kit?

02 December 2021 1 2K Report

I'm getting very low to no yield using this kit. Has anyone used this kit successfully, and if so, are there are modifications or suggestions you used to improve your yield? Thanks!

Megan Ceris Matthews

I have used this kit for extracting fungal(Fusarium spores) or bacterial DNA from soil. The lysis step is critical for yield. For fungi I used 10 minutes bead beating but for bacteria I am using 20-min with the Vortex Genie II 24 prep adapter. Additionally, I elute 30-50 uL twice (total 60-100 uL). I hope this is helpful :)

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