I am in need of at least one or two specimen block(s) [longitudinal, cross sectional) for the comparison of (polysaccharide accumulations') staining efficiacy. If there is anybody who can help, please let me know.
NB: Pompes Disease = Glycogenosis type II or Pompe disease (MIM #232300), is an autosomal recessive lysosomal storage disease due to mutations in the gene encoding acid α-glucosidase (GAA), an enzyme required for glycogen degradation in lysosomes.
(cf. e.g. OPEN ACCESS:
Katona et al. Orphanet Journal of Rare Diseases 2014, 9:17, http://www.ojrd.com/content/9/1/17
Thanking in advance,
regards,
Wolfgang
Dear Wolfgang
I think that the combination of lead citrate uranyl acetate will be OK.
Dear Panagiotis,
I am sorry if I have to tell that staining ultrathin sections is NOT the problem...
I would like to verify an unpublished staining method for POLYCHROMATIC SEMITHIN = ~ 1µm resin SECTIONS for LM.
But nevertheless, I thank you for your comment!
(NB: I only have quite normal specimen blocks containing accumulations of Glycogen or polysaccharide substrate. But would be interested in testing my LM-stain (some are staining semithin sections only with Toluidine Blue, others with e.g. Richardson's AMBF or better MB-Azur II at high pH....)
Best wishes and regards to you and yours and "Hellas"
Wolfgang
Just to explain honestly: the block(s) naturally only for lending....2/4 sections 1 µm thick will be cut from the block(s) and the blocks as well as an image of my staining result(s) will be sent back / provided to a generous "donor/giver" (;.))
Regards, Wolfgang
Due to the fact, that meanwhile I am retired, I will not have the possibility to finish that project I started in 2015, thinking there wiould be any collegial cooperation.... There was none... unfortunately...
But if you look into my "publications" (mainly look for the congress presentations) https://www.researchgate.net/profile/Wolfgang_MUSS/publications
one can imagine from the stained semithin resin sections what the (scientific) purpose should have been....
It might be that I will publish a short article for that staining modification...
Best regards and wishes to all readers and visitors of this my Q-Thread, W.H.M.
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