I am cloning endolysins and need phage phiKZ. I will gladly pay shipping costs. Please email me at [email protected] if you have this phage and will share it. Thank you!
I need the DNA sequence pCP13 cosmid vector. I know that pCP13 was derived from pLAFR1 and have the DNA sequence for pLAFR1 but I don't know the sequence of the kanamycin resistance gene (or where...
29 July 2019 6,065 2 View
My students are isolating Bacillus species from soil that produce antibiotics. I need a transposon mutagenesis method that they can use to identify the biosynthetic pathways for these antibiotics....
07 August 2018 8,688 0 View
Using rat brains; kit says to rapidly freeze brains after initial staining protocol and then section on cryostat. Have too many brains to realistically slice in one day. Once frozen, can brains be...
26 June 2016 3,630 2 View
Hi all, I would like to precipitate DNA from a bacterial lysate using polyethyleneimine (polymin-P). Reading online, I see a range of molecular weights are used. If anyone uses this method...
09 February 2015 1,069 3 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
10 February 2021 5,927 1 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View