I am cloning endolysins and need phage phiKZ. I will gladly pay shipping costs. Please email me at [email protected] if you have this phage and will share it. Thank you!
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
Following your instructions, how to start a Lab https://help.researchgate.net/hc/en-us/articles/14293170669713-How-to-create-and-edit-a-Lab I am not able to find the Lab section on the...
10 May 2023 6,211 0 View
My class is isolating antibiotic-producing bacteria from soil. The majority of the isolates are Bacillus species since we use nonselective media (grown on trypticase soy agar, LB, or potato...
10 June 2021 1,295 3 View
I need the DNA sequence pCP13 cosmid vector. I know that pCP13 was derived from pLAFR1 and have the DNA sequence for pLAFR1 but I don't know the sequence of the kanamycin resistance gene (or where...
29 July 2019 6,226 2 View
My students are isolating Bacillus species from soil that produce antibiotics. I need a transposon mutagenesis method that they can use to identify the biosynthetic pathways for these antibiotics....
07 August 2018 8,804 0 View
Using rat brains; kit says to rapidly freeze brains after initial staining protocol and then section on cryostat. Have too many brains to realistically slice in one day. Once frozen, can brains be...
26 June 2016 3,797 2 View
Hi all, I would like to precipitate DNA from a bacterial lysate using polyethyleneimine (polymin-P). Reading online, I see a range of molecular weights are used. If anyone uses this method...
09 February 2015 1,274 3 View
There was an article posted here about two years ago, with about 50 authors, confirming two earthlike planets from the Kepler mission. Does anyone know the title of that article? I can no longer...
14 January 2014 8,366 3 View
Hello, I have recently sent a request to access the following text book. du Plessis, Hargovan, Bagaric and Harris (2015) Principles of Contemporary Corporate Governance, (3rd edn) Cambridge...
01 January 1970 7,494 1 View
Hello, I am trying to find the plasmids pAW016, pAW068, and pIC333 for transposon mutagenesis of Paenibacillus. I have tried many searching many repositories and have reached out to the authors of...
01 January 1970 2,451 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View