How do I remove all of the cobalt off of TALON beads?

Hi All, I'm regenerating TALON beads in bulk for the lab. As I've done with Nickel beads for years, I washed with water, eluted all the protein, removed imidazole, and then added 0.2M EDTA pH 8.0...

22 September 2020 4,259 1 View

Does anyone know how cosmid pCP13 was constructed? Does anyone know the source of the Kan resistance gene in this cosmid?

I need the DNA sequence pCP13 cosmid vector. I know that pCP13 was derived from pLAFR1 and have the DNA sequence for pLAFR1 but I don't know the sequence of the kanamycin resistance gene (or where...

29 July 2019 6,065 2 View

Why are my mammalian cells dead after cryostorage in liquid nitrogen but not after storage in ultra low (-80) freezer?

I prepare cells for storage according to ATCC guidelines, usually 10% DMSO and 20% FBS. Cells are in cryostorage vials intended for use in liquid nitrogen. Cells are Hek293T, HepG2, HeLa and...

14 May 2019 7,851 3 View

Any recommended methods for transposon mutagenesis of 'nondomesticated' Bacillus isolates from the soil?

My students are isolating Bacillus species from soil that produce antibiotics. I need a transposon mutagenesis method that they can use to identify the biosynthetic pathways for these antibiotics....

07 August 2018 8,688 0 View

Free or low-cost alternatives to NVivo?

Can anyone speak to any psych or health studies that have dealt with qualitative analysis for massive qualitative data sets? Our team does not have funding for NVivo. Does anyone know of any free...

12 December 2017 8,497 3 View

Does anyone have Pseudomonas phage phiKZ that you are willing to share? Will pay shipping costs. Thanks!

I am cloning endolysins and need phage phiKZ. I will gladly pay shipping costs. Please email me at [email protected] if you have this phage and will share it. Thank you!

06 July 2017 8,540 0 View

Why when I overexpress p53 do I observe a decrease in the amount of chromatin precipitated with an anti-p53 antibody?

18 February 2016 7,665 8 View

Which source of do you use polyethyleneimine (Polymin-P) for DNA precipitation from bacterial lysates?

Hi all, I would like to precipitate DNA from a bacterial lysate using polyethyleneimine (polymin-P). Reading online, I see a range of molecular weights are used. If anyone uses this method...

09 February 2015 1,069 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View