Hi,
I am interested to measure oxidative stress (MDA, 4HNE, 8OHdG) and antioxidant (SOD, GPx, Catalase) in SD rat sperm. Does anyone have protocol or reference or expertise to measure these parameters? I really appreciate your cooperation.
Regards,
Check if the authors are here on RG:
Clinics (Sao Paulo). 2013 Jan; 68(1): 93–100.
Fenitrothion induced oxidative stress and morphological alterations of sperm and testes in male sprague-dawley rats
Izatus Shima Taib,I Siti Balkis Budin,I Ahmad Rohi Ghazali,I Putri Ayu Jayusman,I Santhana Raj Louis,II and Jamaludin MohamedI
See our works, maybe you find something interesting. We could measure 8-oxodG (and other DNA modifications) commercially. Please contact directly if you are interested.
Regards
Daniel
Comparison of the absolute level of epigenetic marks 5-methylcytosine, 5-hydroxymethylcytosine, and 5-hydroxymethyluracil between human leukocytes and sperm.
Guz J, Gackowski D, Foksinski M, Rozalski R, Olinski R.
Biol Reprod. 2014 Sep;91(3):55. doi: 10.1095/biolreprod.114.121541. Epub 2014 Jul 24.
PMID: 25061097
Comparison of oxidative stress/DNA damage in semen and blood of fertile and infertile men.
Guz J, Gackowski D, Foksinski M, Rozalski R, Zarakowska E, Siomek A, Szpila A, Kotzbach M, Kotzbach R, Olinski R.
PLoS One. 2013 Jul 12;8(7):e68490. doi: 10.1371/journal.pone.0068490. Print 2013.
Hello! For the MDA determination (Free MDA and Protein MDA) our lab has developed and published a method. See the link below for the abstract. It is a very credible method and works fine with any kind of sample.
Article Method for the simultaneous determination of free/protein ma...
Hope you find it usefull!
Kind Regards
Polyxeni Papadea Could you please share your full text?
Thanks. Does this protocol fit for frozen sperm sample?
You can find attached below the full article. As you will read in the «Sample treatment» section, you can use any kind of sample. You can use a frozen sperm sample but you need to let your sample thawn to RT first. After that, you must isolate the protein fraction of your sample (you can find the detailed process of handling your sample in order to isolate the protein fraction in our paper in the «sample treatment section»). Then you can measure the Protein MDA in the protein fraction, as described.
Check our paper and let me know if you have any further questions!
If you can read some words in Spanish, we have one research article with several protocols to measure oxidative stress in human sperm.
This is the link:
http://www.scielo.org.mx/pdf/ric/v59n1/v59n1a6.pdf
Good look
Dear Md. Mustahsan Billah
There are plenty of good protocols. We recently described a simple and rapid method for measurement of total reductive (antioxidant) capacity with very good precision and accuracy:
Preprint Nitrocellulose redox permanganometry: a simple method for re...
Method Nitrocellulose Redox Permanganometry (NRP/HistoNRP)
Additionally, you can see the references to several good protocols here:
Preprint The Effect of a Color Tattoo on the Local Skin Redox Regulat...
Eg. Hadwans Carbonato-cobaltate assessment of hydrogen peroxide dissociation rate, redox-coupled ORP, standard TBARS assay, DTNB method for thiol assessment,...
Best,
Jan
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