Jan Homolak

11 Questions 16 Answers 0 Followers

Questions related from Jan Homolak

Can you help me identify the tube?

I found a bag of old tubes in the lab that look like spin columns(?). There are no labels so I cannot identify them. I spun some water just to see whether they are porous as they seem to have...

29 April 2023 1,079 1 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

06 January 2021 5,588 1 View

Anybody knows an open-source/free virtual reality morris water maze for humans?

Is there a free software for virtual reality morris water maze tests for humans? Also, are there open arm or other tests in VR format? Thanks! Best, Jan

13 December 2018 242 0 View

C-fos immunofluorescence background difference?

Hi,For some reason I get much higher c-fos background signal in the rat brain for one animal than for the other (see attached photo). The staining protocol is the same for both specimens, as well...

14 August 2017 5,163 3 View

Antigen retrieval methods for frozen sections?

Hi,Do you have experience with antigen retrieval methods for frozen sections? Can the methods be applied to sections that are already cut (rat brain,35um thick)? Do you have any examples of any...

23 June 2017 1,577 4 View

Neuronal activity marker for immunofluorescence?

Hi,I want to evaluate neuronal activity after 15 minutes in my slides using immunofluorescence. I can't use c-fos because mRNA levels peak after 15 min, but protein levels seem to be increased...

28 March 2017 1,561 2 View

Undergraduate and graduate student research grants?

Does anybody know any undergraduate and graduate student research grants in the field of neuroscience?Thanks

25 March 2017 3,062 2 View

Can gut derived BDNF influence the brain?

Can BDNF from gut somehow influence the brain? Can you suggest any papers on this topic? Is it rational to think BDNF could go to the brain through blood and surpass liver? Could BDNF alter gut...

16 March 2017 1,709 4 View

Can you share your protocol for insulin receptor free floating frozen IHC?

I am trying to find the best protocol for free-floating frozen IR IHC.

28 July 2016 8,681 2 View

Can you suggest the best antibody for aspartate aminotransferase that is muscle specific?

Can you suggest the best antibody for aspartate aminotransferase that is muscle specific? I would like to differentiate liver from muscle AST. Also, what would be the best lab method to use for...

26 April 2016 4,574 1 View

Food-specific immunoglobulin G?

Food-specific immunoglobulin G (IgG) testing is currently not a recognized diagnostic tool for food allergy. Do you think there is theoretical evidence to support IgG food antigens testing in...

01 April 2016 8,432 5 View