5/18/17

Dear Lala,

I have attached a method for measuring reducing sugar. I hope this information helps you.

Bill Colonna, Center for Crops Utilization Research, Iowa State University, Ames, IA, USA, [email protected]

The method Bill suggests is a good one.  Here is another paper that compares different methods for reducing sugars.

 Dear W.J. Colonna, Greg Cote 

 Thanks you so much for your  attached file .

 Really helpful for me ........

You may just go to PubMed or PubMed central website and post your question. You will get answers more than you expected.

Reducing sugar protocol u can follow from Miller.

Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31(3), 426–428. https://doi.org/10.1021/ac60147a030

If the DO measurement is for reactor level u can use DO probe which is available with Mettler Toledo.

Why do ask some body to provide you such common protocol? Why don't you get it yourself either from the Net or from standard books on practical biochemistry.  I totally disagree with this type of questions being put up on a Site like RESEARCHGATE. I feel that you should learn to do reference work / literature survey.

Jai:

With all due respect, there are multiple methods for measuring reducing sugars, and it is difficult to know just from reading the protocols in the literature which is most suitable for what purpose. It is perfectly fine to ask others for their advice and input as to which method is best. I agree with you that ResearchGate is no substitute for a proper literature search, but at least in this instance, the question was a legitimate one.  I do tend to agree with you about many of the questions I see posted, particularly those that are clearly from students looking for someone to do their homework for them.

Sincerely,

G L Cote