I am working on isolating mouse heart Purkinje cell for patch-clamp recording. So far it seems to have low percentage of PC in isolated cells and the cell are not healthy.
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...
23 July 2024 4,019 3 View
I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...
22 July 2024 3,975 1 View
Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...
22 June 2024 6,181 1 View
I use a DISCO DAD323 dicing machine to cut my sample,which has 1-2 micrometers SIO2 on its top.However,the SIO2 is broken and fall apart everytime.I tried different cutting speed but didn't work....
20 June 2024 1,131 0 View
I am doing DNA FISH on mice stem cells to detect an endogenous gene location. The expected result should have two signal spots, representing the two copies of the allele. However, what I see is...
06 June 2024 941 1 View
I preprared curcumin-loaded liposome by Thin Film Hydration Method using lecithin, curcumin and beta-sterol. I stained it with phosphotungstic acid and uranium acetate and took TEM images of each....
30 May 2024 6,863 2 View
I carried out low-cycle fatigue tests on a secondary-hardenning ultra-high-strength steel with test condition εmax = 0.9%. It was found that the fatigue life at high temperature was much higher...
29 May 2024 3,176 1 View
I have never success a staining pax7, myoD, eMHY on mouse tissue. (DSHB antibody) When I did a heat-retrieval, staining is success but background was too strong. So I'm not sure if this is a good...
27 May 2024 1,597 1 View
Hello, I am experiencing an error while using Discovery Studio and would like to ask for your assistance in identifying the cause. The details of the error are as follows: [ 22-May-2024 14:59:14...
21 May 2024 4,980 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View