Hello everyone,
I intend to isolate total protein from bacterial cells (in terms of both quality and quantity). My protein is basically an enzyme and hence I will be analyzing its activity. It would be a help if anyone can provide me with a manual protocol for isolation of total protein with intact activity.
Fedora Sutton
As Shruti indicated you need to provide us with more information. What have you already performed? Is this protein being over expressed or is it low abundant?
Check my article, in particular the Materials and methods section of the mini-print for the bacterial lysis protocol.(copy and paste the link into your browser).
http://www.jbc.org/content/261/34/16078.full.pdf+html?sid=301eae80-7cbd-44ec-ae0c-0b2591ec5c0c
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