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Questions related from RAJAL DEBNATH
With open access, you have the liberty of getting more citations because everyone can read it and draw conclusions or relate to their findings, for which a researcher at one end pays the money and...
06 June 2015 7,970 2 View
With the involvement of NGS, most of the researchers have moved to desk than to bench. At bench we understand the biological entity with chemicals and pipettes by monitoring them at specific...
06 June 2015 4,447 11 View
Shared phylotype analysis among sites to visualize spatial effect on microbial communities. How is shared phylotype analysis carried out with geographic distance ( I am aware of generating the...
05 May 2015 3,898 1 View
I am required to do distance decay analysis for my NGS bacterial community along a geographical gradient. What metadata fields are required for the gradient. Will the geographical co-ordinates...
05 May 2015 5,295 2 View
I need help on visualizing a microbial genome sequence obtained from Miseq-paired end reads. I am not sure, when using the tools, how the input file should look. Can anyone help me with generating...
11 November 2014 3,823 3 View
Hello everyone, I intend to isolate total protein from bacterial cells (in terms of both quality and quantity). My protein is basically an enzyme and hence I will be analyzing its activity. It...
08 August 2014 4,065 1 View
I write here to seek help on isolating a lipase gene from a bacterium LTR48. I am trying to amplify lipase from a bacterium which is most likely a Pseudomonas baetica (dendrogram attached). But...
08 August 2014 4,632 3 View
What would you expect if a particular PCR protocol is carried out by setting the annealing temperature of primers at 42 or 45 degree centigrade whereas the actual (calculated) temperature of...
12 December 2013 381 5 View
I am interested in any program for degenerate primer designing other than CODEHOP.
12 December 2013 620 1 View
A forward primer sequence for a 16S rRNA gene on BLAST shows identification with Strenotrophomonas maltophilia having the following output for the first four matches:...
05 May 2013 6,725 1 View
Normal amplification of the particular gene with the same set of primers is routine work for me. But this is how it all got ruined. I am amplifying a set of reaction for sequencing but I am...
03 March 2013 6,291 10 View
RT-qPCR systems have an inbuilt optimized PCR conditions programmed which actually works with most of the primers used. I use an Applied Biosystem 96 well StepONE Plus Platform for comparative...
09 September 2012 9,583 3 View
