Any thoughts on uncultured microbe isolation?

05 June 2015 4,379 11 View

Research in the ERA of OPEN access ??

With open access, you have the liberty of getting more citations because everyone can read it and draw conclusions or relate to their findings, for which a researcher at one end pays the money and...

05 June 2015 7,893 2 View

Can anyone help with the R code for distance decay relationship?

I am required to do distance decay analysis for my NGS bacterial community along a geographical gradient. What metadata fields are required for the gradient. Will the geographical co-ordinates...

04 May 2015 5,187 2 View

Shared phylotype analysis?

04 May 2015 3,789 1 View

How do I visualize the bacterial genome using the ggbio R package/Circo?

10 November 2014 3,732 3 View

Can anyone help with a lipase primer design from genus Pseudomonas?

I write here to seek help on isolating a lipase gene from a bacterium LTR48. I am trying to amplify lipase from a bacterium which is most likely a Pseudomonas baetica (dendrogram attached). But...

07 August 2014 4,556 3 View

Does anyone have knowledge on the total protein isolation for enzyme characterization?

Hello everyone, I intend to isolate total protein from bacterial cells (in terms of both quality and quantity). My protein is basically an enzyme and hence I will be analyzing its activity. It...

07 August 2014 4,003 1 View

PCR by setting annealing point of primers way below the temperature of melting?

What would you expect if a particular PCR protocol is carried out by setting the annealing temperature of primers at 42 or 45 degree centigrade whereas the actual (calculated) temperature of...

11 December 2013 277 5 View

Blast sequence analysis for 16S rRNA gene

A forward primer sequence for a 16S rRNA gene on BLAST shows identification with Strenotrophomonas maltophilia having the following output for the first four matches:...

04 May 2013 6,642 1 View

Removal of secondary bands from PCR.

Normal amplification of the particular gene with the same set of primers is routine work for me. But this is how it all got ruined. I am amplifying a set of reaction for sequencing but I am...

02 March 2013 6,220 10 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View