Commercial materials/surfaces for contact measurement?

Does anyone know of, or use, commercially produced surfaces for contact measurements (control material)? As an example, is there a producer of PTFE discs with highly controlled surface roughness...

09 April 2020 8,806 5 View

What are the pros and cons of running virtual conferences?

What are the pros and cons of running virtual conferences? I ask this question because the current challenging situation with the COVID-19 pandemic has, understandably, meant the cancellation or...

27 March 2020 8,346 17 View

Why is 'Scale' considered a spatial continuum, but not a dimension of space?

Consider the books 'Cosmic View; The Universe in 40 Jumps' by Kees Boeke (1957 - the original inspiration for the others), 'Sizing Up the Universe: The Cosmos in Perspective' by Gott, J. Richard...

27 December 2019 1,030 1 View

How to calculate photovoltaic power output from solar radiation for 100 sites countrywide?

I would like to calculate PV output for 100 sites for one year using hourly solar irradiation and temperature data. Can anyone suggest how I can use a software (e.g. PVSyst, RETScreen or similar)...

21 October 2019 1,061 3 View

Which statistical test for repeated measures ANOVA with multiple patients and biological replicates?

I am analysing human neuronal samples in culture after treatment with different concentrations of NGF. For each patient I am analysing around 30-40 neurons for each of 4 concentrations (including...

05 June 2019 6,162 2 View

Why does my protein destain so quickly?

Hello all, The protein I'm purifying seems to destain very quickly on Coomassie stained SDS-PAGE gels (red box in image). I've heard that proline-rich proteins (like this protein) have a...

06 March 2019 173 6 View

Systemic/social constructionist thinking about trauma?

I'm writing about trauma from a systemic/social constructionist perspective, and wanting to bring in ideas around the 'power threat, meaning framework' that challenge traditional bio-medical...

18 December 2018 2,235 7 View

Any suggestions for a validated measure of healthy behaviors or overall health?

Many of the health scales I have found so far are assessment-based. I am looking for a scale that can be used in continuous data analyses.

10 November 2017 2,705 1 View

DAPI and Hoechst are not staining brightly enough to detect, what other nuclear stain can I use with the Sigma TRAP leukocyte stain kit?

08 September 2016 5,393 3 View

DNA in gram quantities? Where can I purchase/obtain it?

Does anyone know where to obtain pure, 1-10 gram quantities of DNA? Lots of PCRs, or perhaps just bulk DNA purification from cells come to mind, but I was hoping to get pure, controlled sequences...

05 June 2016 7,536 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View