Michael Pujari-Palmer

9 Questions 11 Answers 0 Followers

Questions related from Michael Pujari-Palmer

DAPI and Hoechst are not staining brightly enough to detect, what other nuclear stain can I use with the Sigma TRAP leukocyte stain kit?

We are having problems staining nuclei of human osteoclasts. Dapi and Hoechst staining are too faint to discern clearly, and we are using the TRAP leukocyte stain (Sigma). Even after using tween...

09 September 2016 5,488 3 View

DNA in gram quantities? Where can I purchase/obtain it?

Does anyone know where to obtain pure, 1-10 gram quantities of DNA? Lots of PCRs, or perhaps just bulk DNA purification from cells come to mind, but I was hoping to get pure, controlled sequences...

06 June 2016 7,633 3 View

Does anyone have/know where to purchase Ultrabind US 450 membranes?

We need these for research and can't find them anywhere

05 May 2015 1,715 0 View

Is anyone familiar with Alizarin red MC3T3 washes?

Greetings,I have fixed MC3T3 cultures and incubated with 40mM alizarin red (pH 4.2) for 12 minutes. When rinsed with water, three times for 5 minutes each, the final wash still remains darkly...

10 October 2014 6,852 3 View

Does anyone know of a technique similar to microCT that can distinguish isotopes of the same element?

What technique could one use to create a 3 dimensional image/composition analysis of a material that is made of two isotopes of the same element? For instance, if I make a disc of magnesium, and...

10 October 2014 3,990 7 View

When using cancer cell lines, such as B16F10, or lewis lung cancer (LLC), for chemotaxis studies, what percentage of serum is used to serum starve?

Most articles don't publish the exact percentage of serum. Can anyone direct me towards a reference for any of the following lines: MD-MBA's, B16F10/F1, LLC, PC3?

04 April 2013 2,087 1 View

What chemotherapeutic agents have been developed for inflammatory cancer - ones that target cancer but not local inflammatory cells?

Can anyone recommend chemotherapeutic agents or a good reference comparing efficacy at local injection?

11 November 2012 3,410 8 View

What fixation procedure would you recommend for whole organ fixation that fixes only the cells, not ECM, so I can recover all cells and count them?

I was thinking of using ethanol/methanol. Does anyone have a whole animal alcohol perfusion procedure? Is this possible?

10 October 2012 9,455 5 View

Does anyone have an efficient method of recovering all/most cells from a whole organ?

Edit* I am looking for a way to quantify how many cancer cells are in an organ. I was hoping to isolate all cells from the organ and then do FACS or a cell staining assay. What really matters is...

10 October 2012 9,324 1 View