Does anyone have experience with using the ligation mix is directly to transfect cells?

06 June 2013 1 5K Report

Basically, transformation of ligation mix into E.coli is meant to increase the amount of DNA to be used for transfection. But is it possible and if possible what are the consequences of using the ligation mix directly to perform a transient transfection of cells? Has anyone had previous experience with this strategy ?

Tuval Ben-Yehezkel

Good Question, anyone have experience with this?

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