I'm working on baculovirus expression system (Bac-to-Bac) using Pfast-Bac-1 as a transfer vector , i have cloned my gene (2236) into the vector using SnabI and HindIII Enzymes , and i got positive clones , checked by PCR using Gene forward and Plasmid Reverse primers , however when i carry out double digestion again to confirm the presence of my cloned gene , i got only plasmid band with no gene band in gel electrophoresis , can anybody help me solving this problem , knowing that my enzymes in from NEB and working great . , Thanks is advance

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