What public open software would you recommend for designing HRM primers?

What public open software would you recommend for designing HRM primers?

12 October 2020 3,925 4 View

ISH or immunostaining of GABA neurons?

Hi, Can I reliably label GABA neurons using ISH instead immuno-staining? It is extremely difficult to stain GABA neurons in VLPO and MnPO of hypothalamus by antibody and it seems that ISH is more...

21 September 2020 2,503 4 View

Are there any approved products that can prevent or treat COVID-19 in animals?

10 September 2020 8,643 19 View

Are my birds safe from COVID-19, and is my family safe around my birds?

07 September 2020 4,102 17 View

What tests can be used for a random number generator besides diehard?

What tests can be used for a random number generator besides diehard? Need find tests for my random number generator, based on human-computer interaction.

15 May 2020 7,167 7 View

What is the average depth explored in metal oxide samples through the following characterization techniques: XRD, XPS and Raman spectroscopy?

Obviously, the exact depth evaluated by each characterization technique mentioned (XRD, XPS and Raman spectroscopy) depends on the specific parameters adopted for each analysis. However, in...

15 May 2020 6,418 16 View

How can I add the journal name if your system does not find it in the list?

Hi, I have the published papers (with DOI) in the academic journals that have official ISSN etc. But I can not add and save the names of the journals in my records because your system does not...

26 April 2020 9,394 5 View

What are the storage conditions for phosphines for Staudinger ligation?

Hi all, We would like to purchase 5 mg of phosphine-modified fluorescent dye for site-specific labeling of proteins containing the unnatural aminoacid azide-phenylalanine. We will be labeling...

05 March 2020 3,007 3 View

Metallic materials (more specifically, nickel) present characteristic peaks in Raman spectroscopy analysis?

When evaluating an alumina sample using Raman spectroscopy, characteristic peaks were obtained for certain wavelengths. However, after depositing a thin nickel film via plasma on the surface of...

24 January 2020 8,954 3 View

Does anybody have PDF version of MOE manual?

I will highly appreciate if you can share one for program version 2014 or higher.

28 May 2019 5,615 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View