I am wondering if the claimed LODs and LOQs from the producer are realistic and anyone has confirmed them through the within-laboratory validation/verification procedure.
In order to be more specific, you'll need to know the matrix you want to analyse with this ELISA test. For instance for this analyte the LOD is relatively lower for urine than for example a feed matrix. A quick and easy check is look at the advised calibration and the lowest concentration you calibrate the assay with. When detection limits are claimed that are much lower than the lowest calibrator concentration, then you want to ask the test-kit supplier some questions. How did they find such a low detection limit? Matrix effects are a bit tricky, some matrices need special extraction procedures. When you analyse another matrix than shown in the ELISA product sheet and there is no data published for this matrix elsewhere; then you're probabky stuck with doing some of your own checks (e.g. spike your matrices with known quantities). When the supplier has some of their own validation data (Single Lab / SLV), usually when you ask, they'll be happy to share the results of their single lab study with you. When LOD/LOQ claims are made, also look whether citation is there such as: test (LOD) validation according to SANCO XXX/XXX. Maybe not a straight answer on your question, but hope it helps.
A sensitive and specific ELISA was developed to detect the beta 2-adregenic agonist cimaterol in various matrices such as urine (swine), muscle (chicken), tissue (shrimp), milk and animal feed by Y. Keng Yoon, Theng N. Woan, O. Karen, Z. Michael Asian Pacific Journal of Tropical Disease Volume 4, Issue 3, June 2014, Pages 244
- Badges
- Science method
- Similar topics
- Medicine
- Immunology
- Immunology Techniques
- Immunoassay
- ELISA