I cloned my protein, its size is approximately 60Kd. But its expression shows it was just 20Kd approximately. If there is a possibility of frame shift mutation, can I rectify it by changing the expression vector?
Alexandre Esadze
Run mass-spec your protein seams to have a major band on the gel so highest signal from the mass spec should correspond to it and you also know the expected weight. On gels I have seen the shift in WM for example 10kda protein would show as ~ 15kDa, but I have never seen such a large discrepancy.
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