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Questions related from Vijayakumar Rajendran
I did Tryptophan quenching studies of my truncated protein excitted at 295nm with the emmission range 300-500 nm. I am getting two peaks one at 335nm (one for tryptophan) and other at 380nm. The...
04 April 2017 3,653 25 View
My recombinant protein of 38 kDa has been expressed and it was in soluble fraction (with 10% glycerol, 0.5mM IPTG and 23 degree celcius). but the protein was not binding to the Ni-NTA column. I...
06 June 2016 706 14 View
Recently I read an article about predatory journals saying that they charge money for publishing, fake editorial panel, publishes papers which already been published etc. How far this is genuine?...
11 November 2014 1,753 10 View
I cloned my protein, its size is approximately 60Kd. But its expression shows it was just 20Kd approximately. If there is a possibility of frame shift mutation, can I rectify it by changing the...
06 June 2014 6,461 3 View
Some authors give importance to the impact factor alone and they don't bother with the indexing and citation of their paper. Some authors don't give any importance to impact factored journals...
03 March 2013 2,582 11 View