Vijayakumar Rajendran

5 Questions 44 Answers 0 Followers

Questions related from Vijayakumar Rajendran

Why I am getting a peak at ~380nm in Tryptophan Fluorescence?

I did Tryptophan quenching studies of my truncated protein excitted at 295nm with the emmission range 300-500 nm. I am getting two peaks one at 335nm (one for tryptophan) and other at 380nm. The...

04 April 2017 3,654 25 View

What are the steps to purify the occluded his tag recombinant protein?

My recombinant protein of 38 kDa has been expressed and it was in soluble fraction (with 10% glycerol, 0.5mM IPTG and 23 degree celcius). but the protein was not binding to the Ni-NTA column. I...

06 June 2016 707 14 View

Is the list of predatory journals are genuine? Can we trust those list provided by Jeffrey Beall?

Recently I read an article about predatory journals saying that they charge money for publishing, fake editorial panel, publishes papers which already been published etc. How far this is genuine?...

11 November 2014 1,754 10 View

Does anyone have experience with protein expression shows 20Kd instead of 60Kd in SDS?

I cloned my protein, its size is approximately 60Kd. But its expression shows it was just 20Kd approximately. If there is a possibility of frame shift mutation, can I rectify it by changing the...

06 June 2014 6,462 3 View

What is the advantage for authors in publishing a paper in impact factor journals rather than well indexing journals?

Some authors give importance to the impact factor alone and they don't bother with the indexing and citation of their paper. Some authors don't give any importance to impact factored journals...

03 March 2013 2,583 11 View