Which large sustainable energy demonstration projects are running right now, which have not been reported in a scientific publication yet?

I would like to know which large sustainable energy demonstration projects are running right now, in a country or series of countries, which are too premature to already being subject of a...

03 May 2020 4,174 9 View

R 3D plot with centered axis ?

I want to represent a series of results computed from y = f(x1, x2, x3), with x1, x2 and x3 being in the [-2, +2] range. For now, I use the "scatter3d" package, and an example of a resulting...

21 December 2019 4,986 4 View

How to model proper contact definitions between bodies in ANSYS for rectangular 'pressure vessel'?

Dear all, For a project I am currently working on, the intention is that I calculate the maximum allowable pressure in a rectangular 'pressure vessel'. Of course this shape is not suitable for a...

08 January 2019 7,574 2 View

How do fungicides defeat rust disease?

Also, I would like to ask: 1. How effective are the fungicides? 2. Can rust disease develop resistance? 3. Are there already natural fungicides for rust disease? If you could provide sources to...

16 March 2018 2,135 12 View

What is the effect of dormancy on TTC viability test of embryos?

Will a dormant but living plant embryo always stain red if subjected to TTC? Or can an embryo be so dormant that it will not show dehydrogenese activity?

05 March 2018 7,673 11 View

Does anybody have experience with CATS-small-RNA seq kit from Diagenode?

I am sequencing extracellular vesicle RNA on illumina Nextgen 550, on paper for this kit looks great... but has anybody experience with it?

03 December 2016 2,405 0 View

Does Propidium Iodide have the same fluorescence yield when bound to RNA or DNA?

14 June 2016 8,094 3 View

Troubleshooting Overnight Rest of PBMCs in Serum free Media?

I am working on a protocol to stimulate PBMCs with IFNy to measure transcription activation of genes along the kynurenine pathway using rt-PCR. I want to use serum free media to minimize...

13 June 2016 1,592 3 View

How can I perform mouse cytokine ELISA on high lipid plasma?

Hi All, I'm need to perform an IFNalpha ELISA on mouse plasma samples, however these samples are from mice that received a high cholesterol diet and therefore the plasma is turbid because of the...

11 September 2014 7,970 3 View

Does anyone have experience with Ottix Plus fixation?

In our lab we use Ottix Plus and Ottix shaper dehydration and deparrafination media, as more safe substitutes of Ethanol and Xylene. However, when I was fixing and staining some muscle samples, I...

22 May 2014 8,493 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View