I've been using a lentiviral-based system with a pLenti expression vector in 293T cells and can purify the fragment, but yields are excruciatingly low (~300ug/L). There is quite a bit in the literature about using the pEE system with NSO cells to express antibodies, but will it really increase my yields that much (I've seen published yields of 60mg/L)? I need quite a bit of material for further in-vitro and in-vivo studies. Thanks for any and all help you can provide.
C.H. Brogren
WE have only experience with expression of rat antibodies in HEK and COS cells, but you should ask Modiquest in the Netherlands if they know about NSO expression
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