I have started with evaluation of bull spermatozoa post-thaw with Flow cytometer. I have tried use PI/PSA protocol for simultaneous assessing viability and acrosomal integrity. After first FC session me and  FC technician have doubts about population of cells stained by PI. In plots there are, let say, 2 subpopulations of sperms single possitive to PI. So my question is, if is it possible to assess also DNA integrity on the base of this different PI intensities. I found some mention about assessing of DNA integrity by PI but most authors use SCSA.

Thank you