Does anyone have experience with coating an Elisa plate with sonicated (but previously inactivated) Mycoplasma hyopneumoniae??

Marina Mechler Dreibi @Marina-Mechler-Dreibi

14 February 2022 1 2K Report

I will try a new ELISA protocol in my lab, and have to prepare the coating antigen. However, we only have inactived Mhyo cultures for this purpose. Has anyone tried to sonicate inactivated antigen for protein obtention and have good results? Do you think the inactivation could limit the protein quantification or even inhibit some epitops that could jeopardize my analysis? I appreciate if you guys give some light!

Michael G. Weller

Usually, a coating antigen is a soluble compound. Any larger particles need to be removed by filtration and/or centrifugation. From my experience, particulate materials lead to strange and irreproducible results.

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