Does anyone have experience with Clontechs pRetroX TetOne system?

11 November 2018 0 9K Report

I plan on using the pRetroX TetOne System for Tet-regulatable expression of pre-miRNAs and Protein-coding RNAs. As my cells do not last for many passages I wonder how good the inducibility of puromycin-selected but non-clonal cell-pools is. Does that yield decent expression with low background or is single clone selection and screening a must?

Thank you in advance!

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