I am looking for T and B cells antibodies from Invitrogen, and I would like to know anyone´s opinion.
Why do you have to get antibodies from Invistrigen? There are many other companies selling excellent antibodies. My favourite is Cell Signaling Tech.
Our lab uses antibodies from a couple of companies, also from Invitrogen. I haven't had any problems with them working with T cells.
I am working with several probiotic strains to test the therapeutic effects of their cell-free supernatants (CFS). Most research methods involve treating mammalian cells (for example) with the...
14 November 2023 7,795 0 View
I want to restart the calculation from a defined sub-step within a do-loop. And I get the following warning: *** WARNING *** CP = 226.328 TIME= 13:25:17 Restart for...
10 October 2023 7,511 0 View
Crime and violence have become serious concern of the administration of most countries. It is believed generally that life in prison for a convicted criminal who committed murder is...
04 July 2023 6,520 2 View
Hello everyone, I recently started working on my first systematic review and am struggling now with the "removing duplicates" function in Mendeley. All web tutorials claim that there is a...
22 February 2023 7,781 2 View
This is for a PhD-level Quantitative Research class, and I am confused over what test to run on the G*Power software. It has to be an a priori, but would it be bivariate normal modal, multiple...
26 January 2022 3,081 4 View
We are looking to genotype our mice. Our mice have a mutation in their mitochondrial DNA and we want to know the level of heteroplasmy i.e. % of mutation in their mitochondrial DNA. So we cannot...
09 November 2020 4,421 3 View
Hello, I can't understand why a low yield is obtained with the use of HCl in aldol condensation in acid catalysis for the synthesis of hydroxychalcone.
28 July 2020 8,059 5 View
Hi all, I am researching to find what the major issues mature age students face when going to university either for the first time or after having not been in the environment for decades. Does...
29 June 2020 3,147 7 View
Hi Guys, I would highly appreciate some help/input regarding boolen-stine bootstraping in AMOS. Given that my data violated the multivariate normality assumption I have opted to run bollen-stine...
12 May 2020 2,630 3 View
Hello guys I am looking for positive zeta potential colloidal particles dispersed in water which I can order online. pH around 6 to 7. The size its from 300 nm until 700 nm. Thanks a lot for...
28 March 2020 7,054 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View