Can the Dunn's test be used as an appropriate post-hoc for the G-test?

Research Question: Are red grouse (Lagopus lagopus scotica) a statistically significant proportion of raptor (bird of prey) diets? I am planning on using a series of G-tests (determining whether...

23 February 2021 5,827 3 View

I would like to claim authorship of the article below but have been unable to do so?

Experiences of sleep hygiene education as an intervention for sleep problems in children with developmental disabilities : findings from an exploratory study

07 December 2020 3,607 3 View

Assay with absorbance/fluorescence readout?

I'm setting up a liquid handling robot and need a simple assay to test it. Ideally I would like to mix 3-4 reagents and have a absorbance/fluorescence readout on a plate reader. It's also possible...

11 November 2020 7,645 3 View

Atlas-TI 8: How come some codes with no quotations and no density cannot be deleted?

Hi, I am cleaning and reorganizing some data and there are some codes that I removed all quotations from and still, I cannot remove them. The option to delete them is not available. Does anybody...

28 September 2020 409 2 View

How to submit annotated genomes to NCBI?

Hello, We would like to submit annotated bacteriophage genomes to NCBI but we have a bit of a hard time to fully understand the process. We already created the bioproject and biosamples and...

07 September 2020 715 3 View

If using polymers for flocculant in a waste water treatment plant how often should they have a tox test done?

A wastewater treatment plant is adding dual polymer do reduce TSS, as per their permit they must do tox tests for this. How often and which tests should be done? Thanks

18 March 2020 4,416 3 View

Mir-prefer failed on bam sorting ?

Hello, I am trying to run the pipeline MiR-PREFeR I have run the command pipeline and I have this error after the "Done sam2bam" I have this error : Error occurred when filtering regions and...

04 March 2020 5,930 3 View

Why does my qPCR look like this?

qPCR assays that previously worked perfectly are not working any more and the results are, in a word, baffling (see picture). The amplification appears to work fine until cycle 30 or 35 and then...

20 November 2019 6,650 1 View

Who should be the first author?

After working tirelessly with your graduate student to carry out a research and making serous input in a journal article involving both of you., who should be the first author of this publication?

13 June 2019 4,741 5 View

Can someone suggest a tool for theming qualitative data from a systematic lit review?

I am exploring the composition of an holistic palliative assessment for my dissertation

29 April 2019 4,599 6 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View