28 April 2014 1 10K Report

If so, how expired were your components and how did your results turn out?

More Julie Lynn Horton's questions See All
Why would E. coli have higher results than Fecal Coliform results from the same sample?

During recent sampling for E. coli, most of the sample results were significantly larger then the Fecal numbers. What could possible cause this? Method was membrane filtration reporting in CFU.

18 March 2024 7,848 3 View

What is the cause of streaking and double banding on gels?

I'm working on library prep for ITS NGS using Earth Microbiome Protocol and am getting double banding and smearing on my gels. What might be the cause for this? I should be seeing a band around...

04 March 2024 5,940 1 View

Studies on oceanography - Plastic pollution of our oceans?

I need help, doing a paper on the plastic pollution of our oceans.

10 January 2024 9,313 1 View

Why does Mendeley keeps adding an 'a' to the end of a citation unnecessarily?

Some of my in-text citations references show up with an 'a' at the end (e.g., Smith, 2023a). The author does not have a 2nd publication in my Mendeley library, and I have checked for duplicates....

14 August 2023 1,436 5 View

Ultrapure water for LC-MS/MS: which is best between Evoqua LaboStar PRO TWF UV and Direct-Q® 3 Milli-Q systems?

Dear all, we are doing LC-MS/MS metabolomics and proteomics (research & diagnostics) and I wondered if you had experience with the Evoqua LaboStar PRO TWF water purification system? It's a bit...

19 July 2023 1,532 5 View

How can I add link to open access punlication?

ResearchGate lists multiple open access publications with the message: "Your recent article doesn’t have the full-text yet. Add the full-text of your recent life science work so that others can...

26 May 2023 7,243 1 View

Is there anyone who could provide an insight on Scopus indexed journals for management and social sciences?

It is mandatory that academicians require publication in Scopus Indexed Journal. It is a little tedious in finding out such resources. What was available last year is not available this year. The...

17 May 2023 3,661 2 View

Black aggragates in ELISA assays, what are those?

Hello, End of last year I have developped an indirect ELISA method to detect a couple of caseins in fermentation supernatants. It worked great until a couple of weeks ago. Since then, for each...

26 April 2023 9,506 2 View

Is thematic analysis part of methodology?

I'm new to thesis work and I am thoroughly confused. I understand what thematic analysis is. My professor put down " Results and Thematic analysis" in the table of contents. But it seems wrong. Is...

25 April 2023 611 5 View

If I want to compare two correlations what's the best way to do that in SPSS?

If I have two correlations: one between variable 1 and variable 2; second between variable 1 and variable 3. What's the best way to analyze the data so that I am able to make the assumption that...

22 April 2023 5,343 4 View

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

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